Table 1.

Reactivity of MAbs with receptor chimerasa

MAbReactivity with the following chimera:
4444422224444442RRRRRRHRHHRH
12G5++++
1++++
2++++
8++++
12++++
16++++
17++++
18++++
4G10++++
  • a 293T cells were transfected with plasmids expressing CXCR4 (4444) or the indicated receptor chimeras. Chimeras are designated according to the extracellular domains included in each molecule. Thus, 4222 contains the N-terminal domain of CXCR4 in a CXCR2 background. HHRH contains the second extracellular domain of rat CXCR4 in a human CXCR4 background. Cells were stained with the indicated MAbs and analyzed by FACS. +, reactive; −, not reactive.