Table 1.

Virus-specific cytotoxic activity in the spleens of mice deficient in type I, type II, or both type I and II IFN receptors, or in spleens 129/SvEv of control mice, following infection with 106 PFU of LCMV-Docilea

Day of infectionDilution% CTL activity specific to the indicated viral epitope
129/SvEv miceIFN-α/βR−/−miceIFN-γR−/−miceIFN-α/β-γR−/− mice
GP1NP GP1 NP GP1 NP GP1 NP
61 71 54 81 61 55 47 82 43
352 35 66 41 32 32 64 22
928 16 39 19 14 12 33 8
9 131 17 69 98 28 7 46 15
3 156 42 53 27 7 35 8
9 7 222 3 21 6 10 4
15 1 2 5 1215 1 1 27 13
3 2 3 6 71 2 14 2
9 2 2 2 3 2 16 2
30 1 2 3 4 3 5 4 45
3 2 2 2 2 2 2 3 2
9 2 2 2 2 1 1 2 2
  • a Splenocytes isolated on days 6, 9, 15, and 30 after infection were stimulated in vitro with GP133–41 or NP396–404 peptide-pulsed splenocytes as described in Materials and Methods. The cytolytic activity of restimulated splenocytes cultured at a density of 4 × 106 cells/well was measured in a 51Cr release assay, using MC57G cells pulsed with 10 μg of GP133–41NP396–404 peptide per ml. Restimulated splenocytes were resuspended in 1 ml of medium per culture well, and serial threefold dilutions of effector cells were performed. Lysis of untreated target cells was ≤5% at the highest effector cell/target cell ratio. Results are representative of those from three separate experiments.