Table 1.

CD4+-T-cell epitopes identified in EBNA1

EBNA1 aaaEpitope sequenceNo. of respondersPotential restrictionbKnown restrictionc
DR allele(s)DQ allele(s)
B95.8 sequence
 71–85RRPQKRPSCIGCKGT111, 133, 6
 403–417RPFFHPVGEADYFEY22
 429–448VPPGAIEQGPADDPGEGPST11, 32, 5
 455–469DGGRRKKGGWFGRHR17, 151, 2
 475–489NPKFENIAEGLRALL6DR11
 485–499LRALLARSHVERTTD7[11, 13, 15][1, 3, 6]
 509–528VYGGSKTSLYNLRRGTALAI111, 133, 6
 515–528TSLYNLRRGTALAI5DR1
 519–533NLRRGTALAIPQCRL143
 529–543PQCRLTPLSRLPFGM5[13][3, 5, 6]
 544–563APGPGPQPGPLRESIVCYFM11, 32, 5
 554–573LRESIVCYFMVFLQTHIFAE11, 32, 5
 563–577MVFLQTHIFAEVLKD2DR15
 574–593VLKDAIKDLVMTKPAPTCNI11, 32, 5
 594–613RVTVCSFDDGVDLPPWFPPM2NoneNone
Q/T variant
 424–443DGEPDMPPGAIEQGPADDPG11, 41, 8
 514–533KTSLYNLRRGIALAIPQCRL31
 589–608PTCNIKATVCSFDDGVDLPP31
  • a Data are from screening on the entire B95.8 strain sequence of EBNA1 and on 22 variant peptides incorporating all sequence changes in the Q/T strain EBNA1.

  • b Potential restriction elements based on HLA-DR and -DQ typing of donors. Where the response to a peptide was limited to one donor only, the DR and DQ types of that donor are shown. Where more than one donor responded to a peptide, DR and DQ alleles shared by the responsive donors are shown; if there were no common alleles, then DR and DQ alleles shared by the majority of donors are shown in brackets.

  • c Known restriction elements from the functional analysis of derived clones; note that not all responders to the TSL or NPK epitope carried the relevant DR1 or DR11 allele, so these epitopes may have at least one other restriction element.