Table 2.

Viral transcripts detected in HSV viral particles by RT-PCR and HSV arraya

Gene(s)RT-PCRArray, HSV-1
HSV-1R7023
Vero/IHEp-2/IHEp-2/EHEp-2/EHEp-2/EPosition
UL1A1
UL2A2
UL3A3
UL4+A4
UL5A5
UL6A6
UL7++A7
UL8A8
UL8.5/9A9
UL9A10
UL9.5/10+++++A11
UL11+A12
UL12+++A13
UL13+++A14
UL14A15
UL15+B1
UL15-2/15.5NDNDNDNDB2
UL16B3
UL17B4
UL18++B5
UL19-1++++B6
UL19-2NDNDNDNDB7
UL20+B8
UL20.5+B9
UL21+++B10
UL22B11
UL23B12
UL24B13
UL25B14
UL26NDNDNDNDB15
UL26/26.5C1
UL27++C2
UL27.5/28C3
UL28NDNDNDNDC4
UL29-1+++C5
UL29-2NDNDNDNDC6
UL30-1+C7
UL30-2NDNDNDC8
UL31+C9
UL32C10
UL33C11
UL34++++C12
UL34/35+++++C13
UL36-1+++++C14
UL36-2NDNDNDND+C15
UL36-3NDNDNDNDD1
UL37++D2
UL38D3
UL39D4
UL40+++D5
UL41D6
UL42++++D7
UL43NDNDNDNDD8
UL43/43.5D9
UL44+D10
UL45+D11
UL46+D12
UL47D13
UL48+++++D14
UL49+D15
UL49.5+E1
UL50E2
UL51+++++E3
UL52E4
UL53E5
UL54E6
UL55++E7
UL56+E8
α0-1NDNDNDND+E9
α0-2+E10
α4-1++++E11
α4-2NDNDNDNDE12
LAT/ORFO γ1 34.5E13
Oris-1E14
Oris-2NDNDNDNDE15
US1NDNDNDNDF1
US1/1.5++++F2
US2F3
US3+F4
US4++++F5
US5F6
US6F7
US7++F8
US8F9
US8.5++++F10
US9+F11
US10/11++++F12
US11+++F13
US12+F14
US12-2ND
  • a Vero/I and HEp-2/I, virions purified from homogenized infected cells; HEp-2/E, virions purified from supernatant. R7023 is an HSV-1 mutant lacking the genes US8 through US12. Two or three pairs of primers were designed for the genes that contain exons (i.e., α0, UL15) or for the ORFs longer than 3.5 kb (i.e., UL19-1, UL29, UL30, UL36, α4). The position of the ORFs in the HSV array is also reported (see Fig. 2). ND, not done.