Table 1.

Nucleotide and amino acid differences (in the prME region) between chimeric and parent WT viruses

Virus, cell passageGeneNucleotide no.aNucleotide changeAmino acid changeAmino acid no.bComment
YF/DEN1, Vero P4M389A to GH to R39Present in plasmid
E1109A to GK to R204
E1978–1980CAG to GGCQ to G494Site used to insert NarI
YF/DEN2, Vero P3E1948–1950ATT to GTCI to V484Likely was present in plasmid (9), GenBank accession no. AF038402)
E1978–1980CAG to GGGQ to G494Site used to insert NarI
YF/DEN3, Vero P4E1964C to TA to V489
E1972–1974CAA to GGCQ to G492Site used to insert NarI
YF/DEN4, Vero P3 (uncloned)M400G to AA to T43
E508G to AV to I4
E666A to TL to F56
E1807C to TH to Y437
E1978–1980CAA to GGCQ to G494Site used to insert NarI
YF/DEN4, Vero P8 (large plaque)M400G to AA to T43
E666A to TL to F56
E1327C to AH to N277
E1595A to GN to S366
E1807C to TH to Y437
E1978–1980CAA to GGCQ to G494Site used to insert NarI
YF/DEN4, Vero P8 (small plaque)M400G to AA to T43
E666A to TL to F56
E1978–1980CAA to GGCQ to G494Site used to insert NarI
  • a From beginning of the prME gene.

  • b From N terminus of the protein (M or E).