Table 2.

Nonmammalian cells divide at rates which are sufficient to promote nuclear entry of the MoMLV PIC

Cell lineDoubling time (h)aEfficiency of MoMLV-based vector transduction (% relative to that of D17 cells)bRT product (% relative to that of D17 cells)c
D1730100100
ZF44011
FHM29<0.0010.1
High-536<0.0011
Mos-5532<0.0011
XPK248<0.0010.75
Sf960<0.001ND
  • a Cells were plated into multiple dishes. Duplicate plates were trypsinized, and the cells were counted after treatment with trypsin every 24 h for 4 days. The doubling times were determined between days 2 and 4, the period in which the cells were exposed to virus for transduction assays.

  • b Transduction by MoMLV-based vectors pseudotyped with either the GALV, RD114, or VSV-G envelope proteins.

  • c Detected after exposure to MoMLV-based vectors. ND, not done.