Table 2.

Transduction of polarized human airway epithelia by AAV2 and AAV6 vectorsa

Vector pseudotypeAge of epithelia (days)No. of vector particles/cellβ-Gal FFU
AAV24500
450017
11500, 0
115003, 3
AAV6450212
4500640
1150108, 388
11500485, 420
  • a AAV2 and AAV6 pseudotype vectors, Rep2Cap2Genome2 and Rep2Cap6Genome2, encoding nucleus-localizing β-Gal were used to infect airway epithelia (106 cells seeded per transmembrane culture) by apical exposure for 4 h. Epithelia were fixed and stained for β-Gal expression 5 days later. Primary bronchial airway cells used to generate epithelia were derived from two CF individuals.