Table 1.

Most nonmammalian cells tested were resistant to transduction by MoMLV-based vectors but susceptible to transfection by vector plasmids

Cell lineTransfection by plasmidaTransduction by vectorb:Infection by VSV-GFPb
pLNCZpLNCGLNCG(10A1)LNCZ(amphotropic)LNCZ(GALV)LNCG(MDEV)LNCG(RD114)LNCZ(VSV-G)
High-5++ND<2<1<1<2<2<10NA
SF9++ND<2<1<1<2<2<10NA
S2+ND<2<1<1<2<2<10ND
XPK2++<2<1<1<2<2<10<200
FHM++++<2<1<1<1ND<109 × 106
ZF4++<2<12 × 103 <11 × 104 5 × 104 7 × 104
Mos-55++++++<2<1<1<1ND<102 × 109
D17NDND1 × 105 1 × 105 1 × 105 NDND6 × 106 2 × 109
HT-1080NDNDNDNDND1 × 104 1 × 106 NDND
  • a Percentages of GFP- or β-galactosidase-positive cells were determined 2 to 4 days after transfection. Results are means of two experiments. +, <1% but >0%; ++, 1 to 10%; +++, >10%; ND, not done.

  • b Foci of GFP- or β-galactosidase-positive cells were counted 2 to 4 days after infection, and results are expressed as FFU per milliliter of virus added to the cells. Results are means of two to four experiments. ND, not done; NA, not applicable (gfp expression was difficult to distinguish due to the autofluorescence of the cells).