Table 2.

Adenovirus vector-induced reactivation of HSV-1 in TG cell cultures

TreatmentReactivation frequency in expt:Total no. reactivated/ total culturese (mean % ± SD)
123
Negative controls
  Mock1/122/121/124/36 (11 ± 5)
  Ad.C-GFPa 2/121/12NDb 3/24 (13 ± 6)
  Ad.C-rtTA onlya 2/122/12ND 4/24 (17 ± 0)
  Σ Backgroundc 5/365/361/1211/84 (12 ± 3)
Test vectorsd
  Ad.T-ICP010/1211/1210/1231/36∗∗ (86 ± 5)
  Ad.T-n2121/121/12ND 2/24 (8 ± 0)
  Ad.T-ICP410/1210/1211/1231/36∗∗ (86 ± 5)
  Ad.T-OBP1/120/12ND 1/24 (5 ± 5)
  Ad.T-OBPψ0/120/12ND 0/24 (0 ± 0)
  Ad.T-VP1611/1212/1212/1235/36∗∗ (97 ± 5)
  Ad.T-VP16Δ2/121/12ND 3/24 (13 ± 6)
  • a Cultures were superinfected with 10 PFU of Ad.C-GFP or Ad.C-rtTA/cell and incubated in TG cell culture medium containing 3 μM DOX.

  • b ND, not determined.

  • c Σ Background, the sum of the frequencies of reactivation observed in negative-control cultures that were either mock-superinfected or superinfected with Ad.C-GFP or Ad.C-rtTA only.

  • d Cultures were cosuperinfected with 10 PFU of Ad.C-rtTA/cell and 40 PFU of TRE adenovirus vector/cell and incubated in TG cell medium containing 3 μM DOX.

  • e ∗∗, P < 0.00001 , H0: reactivation frequency in treatment group is equal to the background, based on comparison of reactivation frequencies by Fisher's exact test.