Table 1.

Generation of cross-reactive neutralizing antibodies in rabbits

gp140AnimalSpecific neutralizationa
91US05492US65792US66092HT59391US05692US714
50%80%50%80%50%80%50%80%50%80%50%80%
Unmodified SF162 A1
A2 + +
A3 +
A4 +
A5 + + + +
A6 +
Modified ΔV2 A7+ + + + + + +
A8 + + + ++ + + +
A9 + + + + + + + +
A10 + + + + + +
A11
A12
  • a Neutralizing activity was evaluated at 1:10 dilution, taking into consideration the nonspecific neutralization recorded with sera collected from animals vaccinated with the DNA vector alone (see Materials and Methods for details). −, 50% specific neutralization was not recorded; +, 50 or 80% specific neutralization was recorded. Results are from three independent neutralization experiments.