Table 2.

EBV latent protein expression and promoter usage in Akata virus-infected clones of BL28, DG75, and Ramos cell linesa

Cell line EBNA-1EBNA-2LMP1QUKY3UK
Raelb ++
Rajib +++++
BL28
BL28 cl-1+++++
BL28 cl-2+++++
BL28 cl-3+++++
BL28 cl-4+++++
BL28 cl-5b +++++
BL28 cl-6+++++
Ramosb
Ramos cl-1+++++
Ramos cl-2+++++
Ramos cl-3+++++
Ramos cl-4+++++
Ramos cl-5+++++
Ramos cl-6b +++++
DG75
DG75 cl-1+++++
DG75 cl-2b +++++
  • a EBV protein expression was verified by immunoblotting, and promoter usage was verified by RT-PCR. Immunoblotting and RT-PCR were repeated at least twice for each sample. PCR was performed 3 to 4 months after infection.

  • b These lines were also examined for either Qp or W/Cp usage by using additional sets of nested primers that distinguish the two transcripts.