TABLE 1

Frequencies of mutations in cell-free virus within the E2 region

WT residueMutation(s) (frequency [%]) at passage:a
0479
V414V (100)V (100)V (99), A (<1)V (99), A (<1)
N415N (100)N (100)N (70), D (27), S (1), T (<1), G (<1)D (79), N (16), S (3), T (<1), G (<1)
N417N (100)N (99), S (<1)N (98), S (1)N (87), S (12)
G418G (100)G (100)G (99), A (<1)G (99), A (<1)
I422I (100)I (100)I (100)I (99), V (<1)
D431D (100)D (100)D (99), G (<1)D (100)
I438I (100)I (100)I (100)I (99), V (<1)
Y443Y (100)Y (99), H (<1)Y (100)Y (100)
F447F (100)F (100)F (100)F (99), L (<1)
M456M (100)M (99), V (<1)M (100)M (100)
E482E (100)E (100)E (100)E (99), G (<1)
N534N (100)N (100)N (99), S (<1)N (99), D (<1)
L540L (100)L (98), P (1), V (<1), I (<1)L (99), P (<1), V (<1)L (99), P (<1), V (<1)
L541L (100)L (100)L (99), S (<1)L (100)
  • a Extracellular virus was obtained from the selected passage numbers from cultures of Huh7.5 cells initially infected with virus in the presence of 9.8 μg/ml MAb24 with serial passage of infected cells and extracellular virus.