Table 1.

Determination of the MF in DEN-treated transgenic mice

Age (days)GenotypeAnimal IDaTotal no. of PFU (105)bNo. of mutant PFUcMF (10−4)Mean MF ± SD (10−4)
30 ATX21014.721212.572.01 ± 0.67
21082.12552.59
21334.561022.24
21346.521021.57
21363.39361.06
WTd 20843.13762.431.83 ± 0.38
20854.12691.67
20964.20651.55
20975.851161.98
20984.78721.51
90 ATX20553.581393.882.39 ± 1.33
20582.95581.97
21283.02401.32
WT20523.87822.121.79 ± 0.70
20563.13712.27
21274.23420.99
240ATX20592.54873.432.49 ± 0.75
20612.80702.50
20622.77953.43
20702.07472.27
20824.011042.59
21375.94951.60
21393.87631.63
WT20603.58942.621.99 ± 0.46
20635.281041.97
20652.57391.52
20672.49562.25
21323.87611.58
WT (untreated)e 0.306 ± 0.28
  • a ID, identification.

  • b Calculated from dilutions incubated at 37°C.

  • c Isolated mutants replated and incubated at 24°C to verify phenotype.

  • d WT, wild type.

  • e MF value reported for untreated WT mice at 240 days old by Madden et al. (35).