Table 2.

Efficiency of replication of HSV-1 strain 17+ and ICP0 mutants dl1403 and M1 in HFL, NT2, and hNT cellsa

Virus and input MOIbTiter (PFU/ml) at 36 h in cell type:Fold reduction
HFLNT2HFL vs. NT2 at 36 hHFL vs. NT2 at 48 hNT2 vs. hNT at 36 h
17+
 0.012.0 × 108 4.3 × 105 4652443.0
 0.18.7 × 107 8.7 × 106 10121.6
 17.1 × 107 2.8 × 107 333.7
dl1403
 53.8 × 106 5.5 × 104 69774.7
 508.2 × 106 5.1 × 105 16101.2
 5007.6 × 106 5.1 × 105 15102.5
M1
 0.011.5 × 107 5.7 × 103 2,66726,0000.3
 0.17.1 × 107 6.1 × 104 1,1645620.46
 17.3 × 107 1.4 × 105 514170.57
  • a Viruses 17+, dl1403, and M1 were used to infect the three cell types at the indicated multiplicities (calculated on the basis of titration in U2OS cells). Cells and medium were harvested 36 and 48 h later and sonicated, and the virus progeny was titrated on U2OS cells. Actual titration figures are given for the 36-h samples. The fold reduction of the virus titers in NT2 compared to HFL cells are shown at the two time points. Note that the yields of wild-type virus in HFL cells do not decrease with multiplicity because rapid viral replication ensures all the cells become infected during the time course of the experiment. The high figure for the reduction of M1 replication at the lowest multiplicity in NT2 cells was due to further replication of the virus between 36 and 48 h in HFL cells but not in NT2 cells. Comparative yields of the viruses in NT2 and hNT cells from a separate experiment are given in the last column. vs., versus.

  • b MOI, multiplicity of infection.