Table 1.

Replication of HSV-1 strain 17+ in HFL, NT2, and hNT cellsa

Input MOIbTiter (PFU/ml) at 36 h in cell type:Fold reduction
HFLNT2hNTNT2hNT
509.4 × 107 7.4 × 107 1.2 × 107 1.37.8
106.3 × 107 2.7 × 107 1.0 × 107 2.46.3
16.2 × 107 1.9 × 107 5.6 × 106 3.011
0.17.8 × 107 1.1 × 106 6.8 × 105 70114
0.017.3 × 107 1.8 × 105 5.9 × 104 4141,228
  • a The three cell types were infected with HSV-1 strain 17+ at the indicated multiplicities, and then the cells and medium were harvested after 36 h and sonicated. The virus progeny was titrated on BHK cells. The fold reduction of the virus titers in NT2 and hNT cells compared to HFL cells is shown. Note that the yields of virus in HFL cells do not decrease with multiplicity because rapid viral replication ensures all the cells become infected during the time course of the experiment.

  • b MOI, multiplicity of infection.