Table 1.

Dengue virus infection induces DC maturation and activationa

DCsSurface molecule expression (MFU)b
CD83 (n = 6)CD40 (n = 5)CD80 (n = 6)CD86 (n = 6)HLA class I (n = 6)HLA class II (n = 6)
Mock infected (control)4 ± 11,053 ± 10949 ± 965 ± 1923 ± 384 ± 20
Dengue virus infected27 ± 3c 1,771 ± 186c 159 ± 22c 508 ± 105c 64 ± 13c 187 ± 33c
Uninfected (bystander)46 ± 6c,d 1,862 ± 159c 210 ± 26c,d 1,063 ± 171c,d 114 ± 20c,d 202 ± 37c
Dengue virus infected in the presence of IFN-γ (100 U/ml)25 ± 41,954 ± 224e 240 ± 23f 936 ± 64f 107 ± 13f 233 ± 34f
  • a Cell surface activation marker expression in CD1a+ DCs at 48 h following dengue virus infection in the absence or presence of IFN-γ.

  • b Expressed as specific mean fluorescence intensity units (MFU) calculated by subtracting the MFU obtained with the corresponding isotype control. Data are presented as the mean ± standard error of the mean.

  • c P ≤ 0.01 compared to mock-infected (control) DCs.

  • d P ≤ 0.01 compared to dengue virus-infected DCs.

  • e P = 0.02 compared to dengue virus-infected DCs.

  • f P < 0.005 compared to dengue virus-infected DCs.