Table 1.

Qualitative examination of CPE in Vero cellsa

FlavivirusVectorCPE of LgtE-D3b
Dengue virus type 4Mosquito+
Japanese encephalitis virusMosquito+
Yellow fever virusMosquito+
Langat virusTick
Powassan virusTick
  • a Cells were grown until 60 to 70% confluent, washed with cold PBS, and incubated on ice with 20 μM LgtE-D3. Cells were challenged with virus at a multiplicity of infection of 0.5 and incubated again for 30 min on ice. The cells were then supplemented with 2 ml of Dulbecco's modified Eagle's medium containing 2% (vol/vol) fetal bovine serum (viral maintenance medium) and incubated at 37°C in 5% carbon dioxide.

  • b Presence (+) or absence (−) of CPE compared to control cells.