Table 2.

CD8+ T lymphocytes protect against lethal Ebola virus challenge

Cells or serum transferred Amt transferred No. of survivors/total after challenge with:
300 LD50s3,000 LD50s
Unfractionated T cells8 × 106  cells20/20 10/10
1 × 106  cells 16/20 8/10
1 × 105  cells 3/20 1/10
1 × 104  cells 1/20 0/10
CD8-enriched T cellsa 1 × 106  cells 17/20 9/10
CD4-enriched T cellsb 1 × 106  cells0/20 NDe
MLR control T cellsc 8 × 106  cells 1/20 0/10
Naive control T cellsd 8 × 106  cells0/20 0/10
Ebola virus NP serum1 ml 0/20ND
Lassa virus N serum1 ml 0/20ND
  • a Combined results from different experiments, in which the CD8-enriched cultures were 94 to 96% CD8+ cells and <3% CD4+ cells.

  • b Combined results from different experiments, in which the CD4-enriched cultures contained 95 to 98% CD4+ cells and 2 to 3% CD8+ cells.

  • c MLR, mixed lymphocyte reaction. Control cells specific for allogeneic BALB/c cells and approximately 80% CD8+.

  • d Naive control cells from unvaccinated C57BL/6 mice that were stimulated in vitro with the Ebola virus NP peptide.

  • e ND, not determined.