Table 2.

Preincubation experiments to show that all ΦK1-5 particles contain both tail proteinsa

PhageTiter
ΦK1-5
 Preincubated with 235065.2 × 108
 Titered on 23506 after preincubation3.1 × 106
 Titered on 23503 after preincubation3.7 × 106
 Preincubated with 235035.2 × 108
 Titered on 23506 after preincubation6.8 × 106
 Titered on 23503 after preincubation6.4 × 106
ΦK5
 Preincubated with 235064.0 × 108
 Titered on 23506 after preincubation8.5 × 105
 Preincubated with 235034.0 × 108
 Titered on 23506 after preincubation3.3 × 108
ΦK1E
 Preincubated with 235067.7 × 108
 Titered on 23503 after preincubation6.8 × 108
 Preincubated with 235037.7 × 108
 Titered on 23503 after preincubation3.0 × 106
  • a Preincubation of ΦK1-5 with either ATCC 23503 (K1) or ATCC 23506 (K5) for 5 min results in roughly 100-fold loss of phage particles when titered on either strain. If the phage preparation consisted of two populations that each contained just one tail protein, then titers after the incubation would be reduced only on the strain that was used in the preincubation. ΦK5 titers are reduced by preincubation with a permissive host (23506) but not with a nonpermissive host (23503). ΦK1E titers are also reduced only by preincubation on a permissive host (23503) and not a nonpermissive host (23506).