Table 1.

Primers used for the detection of latent and lytic transcripts by quantitative RT-PCR

Target and primer Sequence
orf29a
 tac29-5F5′-CCCGGAGGACGGTCCA
 tac29-68R5′-CCCCGAATGCTCTGTTCTTATT
 tac29-22T5′-FAM-CTCGCTGATGTGCGCAACATGCT-TAMRA
k-cyclina
 lat-335R5′-CAGGTTCTCCCATCGACGA
 TAQ-F15′-AGGCAGCTGCGCCACGAAGCA
 lat-294T5′-FAM-TAGCGTACTCTCGCGGCCCAGC-TAMRA
GAPDHa
 gapdhF5′-GAAGGTGAAGGTCGGAGTC
 gapdhR5′-GAAGATGGTGATGGGATTTC
 gapdhP5′-VIC-CAAGCTTCCCGTTCTCAGCC-TAMRA
orf50b
 orf50s15′-CACAAAAATGGCGCAAGATGA
 orf50a45′-TGGTAGAGTTGGGCCTTCAGTT
 orf50p5′-FAM-AGAAGCTTCGGCGGTCCTG-TAMRA
vGCR
 gerF5′-TGGCCCAAACGGAGGATCCTAG
 gerR5′-AGTTTCATTCCAGGATTCATCATC
 gerP5′-FAM-AGAAGATGGTTAGGAAATCCTCGGC-TAMRA
  • a Data from Dittmer et al. (10).

  • b Data from Chang et al. (5).