Table 1.

Infectivity of transfected T-BAC DNAsa

GenotypeNo. of exptsbNo. of plaques/transfection
RangeMean
Wild type 74–642326
Δ1226NAc 0
Δ122R710–648223
  • a HFF cells were transfected with T-BACwt, T-BACΔ122, or T-BACΔ122R and monitored for plaque development (see Materials and Methods).

  • b Data are from the indicated number of representative experiments out of a larger number of trials using multiple preparations of viral and BAC DNA over a 6 month period.

  • c NA, not applicable (no preparation of T-BACΔ122 DNA yielded plaques which could be recognized either morphologically or by GFP expression, even after 4 weeks of culture).