TABLE 1

Definition of peripheral blood and tonsillar lymphocyte subsetsa

Subset nameDefinition
T cellsCD3+
    CD4+ T cellsCD3+ TCRγδ CD4+ CD8
        Naive cellsCD3+ TCRγδ CD4+ CD8 CD45RA+ CCR7+ CD27+
        Memory cells
            TCMCD3+ TCRγδ CD4+ CD8 CD45RA CCR7+ CD27+
            TEMROCD3+ TCRγδ CD4+ CD8 CD45RA CCR7
            TEMRACD3+ TCRγδ CD4+ CD8 CD45RA+ CCR7
            TFHCD3+ TCRγδ CD4+ CD8 CD45RA CXCR5+
            TH1CD3+ CD4+ CD8 CD45RA CD25low CCR6 CXCR3+ CCR4
            TH2CD3+ CD4+ CD8 CD45RA CD25low CCR6 CXCR3 CCR4+
            TH1TH17CD3+ CD4+ CD8 CD45RA CD25low CCR6+ CXCR3+ CCR4
            TH17CD3+ CD4+ CD8 CD45RA CD25low CCR6+ CXCR3 CCR4+
            TregCD3+ CD4+ CD8 CD45RA CD25high CD127−/low
    CD8+ T cellsCD3+ TCRγδ CD4 CD8+
        Naive cellsCD3+ TCRγδ CD4 CD8+ CD45RA+ CCR7+ CD27+
        Memory cells
            TCMCD3+ TCRγδ CD4 CD8+ CD45RA CCR7+ CD27+
            TEMROCD3+ TCRγδ CD4 CD8+ CD45RA CCR7
            TEMRACD3+ TCRγδ CD4 CD8+ CD45RA+ CCR7
B cellsCD19+
    TransitionalCD19+ CD21high CD24+ CD27 CD38+
    Plasma cellsCD19+ CD21high CD24 CD27+ CD38+
    GC cellsCD19+ CD21high CD24 CD38high
    Naive cellsCD19+ CD21high CD38low CD27 IgM+
    Memory cells
        IgM+/non-CSM cellsCD19+ CD21high CD38low CD27+ IgM+
            IgM-only cellsCD19+ CD21high CD38low CD27+ IgM+ IgD
            Natural effectorCD19+ CD21high CD38low CD27+ IgM+ IgD+
        CSM B cellsCD19+ CD21high CD38low CD27−/+ IgM
            IgG+ cellsCD19+ CD21high CD38low CD27−/+ IgM IgD IgA IgG+
            IgA+ cellsCD19+ CD21high CD38low CD27−/+ IgM IgD IgA+ IgG
  • a TCM, central memory T cells; TEMRO/TEMRA, CD45RO+/CD45RA+ effector memory T cells; TFH, follicular helper T cells; TH, helper T cells; Treg, regulatory T cells; non-CSM, non-class-switched memory; CSM, class-switched memory; GC, germinal center.