Table 1.

Oligonucleotides used for amplification of HCV RNAs from cell line 9-13

NameSequence
3′-Ligaa ACT AGT GAC TAC GAC GTG ATA GTG CCA GTC TGA CGA AGC TTG ACG ATG
1/3′CAT CGT CAA GCT TCG TCA GAC TGG
2/3′CAC TAT CAC GTC GTA GTC ACT AGT
5′-Ligab GGG CGA AUU GGG UAC CGG GCC CCC CCU CGA GA CUA GUG ACU ACG ACG UGA UAG UGC CAG UCU GAC GAA GCU UGA CGA UGU CUA G
S-Liga1ACT AGT GAC TAC GAC GTG ATA GTG C
S-Liga2GCC AGT CTG ACG AAG CTT GAC GAT G
Aneo3′PmeACA AGA GTT TAA ACT CAG AAG AAC TCG TCA AGA AG
A220AGG CAT TGA GCG GGT TGA TCC
A325GAC CTC CCG GGG CAC TCG CAA GCA CC
A349GTG CTC ATG GTG CAC GGT CTA CGA
A3802GAG AGT AGG CTC CCC CTG CTG TC
A4919AGC ACA GCC CGC GTC ATA GCA CTC G
A9386TTA GCT CCC CGT TCA TCG GTT GG
A9412CAG GAT GGC CTA TTG GCC TGG AG
A9605Sca-SpeTGC ACT AGT AGT ACT TGA TCT GCA GAG AGG CCA GTA TC
SEMCV-PmeIACA AGA GTT TAA ACA GAC CAC AAC GGT TTC CCT CTA GC
ST7/Hind1-28GCT AAG CTT CGT AAT ACG ACT CAC TAT AGC CAG CCC CCG ATT GGG GGC GAC ACT CC
S59TGT CTT CAC GCA GAA AGC GTC TAG
S8467GTA ATA CCC TCA CAT GTT ACT TGA
S9392CCA GGC CAA TAG GCC ATC CTG
S9503CCT TTG GTG GCT CCA TCT TAG
  • a 3′ end blocked with an amino link.

  • b RNA synthesized by in vitro transcription.