Table 1.

PKR phosphorylation sitesa

Protein and amino acidSequence
eIF2α L S E LS R R R I
Tat G Q S S T H Q V S
p53 E G P D S D
PKR
 Ser 242 K A K R SL A P R
 Thr 255 D M K E T K Y T V
 Thr 258 E T K Y TV D K R
 Ser 83 K K A VS P L L L
 Thr 88, 89, 90 P L L LT T T N S
 Thr 446 D G K R T R S K G
 Thr 451 R S K G TL R Y M
  • a Sequences surrounding known PKR phosphorylation sites within PKR itself and in its substrates eIF2α, human immunodeficiency virus type 1 Tat, and p53 are aligned. Phosphorylated amino acids are in boldface. Uncharged (L and V) and basic (K or R) amino acids within 1 to 3 residues of phosphorylation sites are underlined.