Table 3.

Positive responses detected against 14 Mamu-A*01-bound peptides by ELISPOT in SIV-infected Mamu-A*01+ macaquesa

Peptide no.Peptide nameSequenceResponse to peptide at concn (μM)
96031950249511495115
101101011101110
1  Env CL8 CAPPGYAL+++
2  Pol LV10 LGPHYTPKIV++
3 Pol_EA11EAPQFPHGSSA+
4  Env CL9 CAPPGYALL+++++
5 Env_ST10SPPSYFQTHT
6  Pol_SV9621 STPPLVRLV+++
7  Pol_SV9 QVPKFHLPV++
8  Env_TL9 TVPWPNASL+++++++
9 Vpx_GL10GPPPPPPPGL+
10Pol_IL10IYPGIKTKHL+
11Pol_SL8STPPLVRL+
12Pol_YI9YTPKIVGGI
13 Gag_LF8 LAPVPIPF+++
14Rev_DL10DPPTNTPEAL+
15Pol_GM10GSPAIFQYTM
16Gag_LA9LAPVPIPFA+
17Vpx_HV10HLPRELIFQV
18Env_SI9SWPWQIEYI+
19Pol_MI8MTPAERLI
20Pol_QF10QMPRQTGGFF
21Pol_GT9GSPAIFQYTntnt
22 Gag_CM9 CTPYDINQM+++++++++
23Vif_LL8LTPERGWLntnt
24Gag_VL8VVPGFQALntnt
25Vif_VI8VTPDYADI
26 Vif_QA9 QVPSLQYLA++
27 Tat_TL8 TTPESANL++++
28Vif_RW9RIPERLERWntnt
29Env_AV8AAPTSAPV
30 Gag_QI9 QNPIPVGNI+++
31Env_YI9YVPCHIRQIntnt
32 Env_ST9 SSPPSYFQT++nt+nt+
33 Vpx_II11 IPPGNSGEETI+nt+nt
34Gag_VT10VNPTLEEMLT+
35Env_IT9ITPIGLAPTnt+nt
36 Gag_LW9 LSPRTLNAW++nt+nt
37 Pol_SV9692 SGPKTNIIV+nt+nt
  • a Since the Env_CL8 and Env_CL9 peptides overlap, we consider this a single positive response. Mamu-A*01-bound SIV-derived peptides were considered positive responders (shown in boldface) if peptides yielded two or more significant responses (+) by ELISPOT in the four SIV-infected animals tested. Each column of data is from a separate experiment. Not all peptides were tested in each animal (nt). Peptides were tested at 1 and 10 μM concentrations.