Table 4.

Recall CTL responses detected against 16 Mamu-A*01-bound peptides by CTL assays in SIV-infected Mamu-A*01+ macaquesa

Peptide no.Peptide nameSequencePositive responses/ no. of tests
950249511495115
1 Env_CL8 CAPPGYAL2/30/11/1
2 Pol_LV10 LGPHYTPKIV1/31/11/1
3Pol_EA11EAPQFPHGSSA0/30/11/1
4 Env_CL9 CAPPGYALL3/40/11/1
5 Env_ST10 SPPSYFQTHT1/31/11/1
6Pol_SV9621 STPPLVRLV0/30/11/1
7 Pol_QV9 QVPKFHLPV1/30/11/1
8Env_TL9TVPWPNASL0/30/11/1
9Vpx_GL10GPPPPPPPGL0/30/10/1
10Pol_IL10IYPGIKTKHL0/31/1nt
11Pol_SL8STPPLVRL0/31/1nt
12 Pol_YI9 YTPKIVGGI2/30/1nt
13 Gag_LF8 LAPVPIPF3/31/1nt
14Rev_DL10DPPTNTPEAL0/30/1nt
15 Pol_GM10 GSPAIFQYTM3/41/1nt
16 Gag_LA9 LAPVPIPFA3/41/1nt
17Vpx_HV10HLPRELIFQV0/30/1nt
18Env_SI9SWPWQIEYI1/30/1nt
19 Pol_MI8 MTPAERLI2/21/10/1
20 Pol_QF10 QMPRQTGGFF1/21/11/1
21Pol_GT9GSPAIFQYT0/20/10/1
22 Gag_CM9 CTPYDINQM0/2b 1/11/1
23Vif_LL8LTPERGWL0/20/10/1
24Gag_VL8VVPGFQAL0/20/10/1
25 Vif_VI8 VTPDYADI1/21/10/1
26 Vif_QA9 QVPSLQYLA2/21/10/1
27 Tat_TL8 TTPESANL1/21/10/1
28Vif_RW9RIPERLERW0/20/10/1
29 Env_AV8 AAPTSAPV2/2ntnt
30Gag_QI9QNPIPVGNI1/2ntnt
31Env_YI9YVPCHIRQI0/2ntnt
32Env_ST9SSPPSYFQT0/2ntnt
33Vpx_II11IPPGNSGEETI0/2ntnt
34 Gag_VT10 VNPTLEEMLT2/2ntnt
35Env_IT9ITPIGLAPT0/2ntnt
36Gag_LW9LSPRTLNAW0/2ntnt
37Pol_SV9692 SGPKTNIIV0/2ntnt
  • a Since the Env_CL8 and Env_CL9 peptides overlap, we consider this a single positive response. Two-week in vitro-stimulated PBMC from three SIV-infected Mamu-A*01+macaques were tested against each of the 37 identified peptides. The number of positive CTL responses per total number of assays tested for each peptide is listed. 51Cr release assay responses were considered positive if the percentage of specific lysis was >15% over that of an irrelevant peptide. Peptides were considered positive responders (shown in boldface) if 50% or more of the assays (were two or more assays were conducted) in a given animal were positive or if two or more assays from any of the three animals tested combined were positive. Effector-to-target cell ratios in the 51Cr release assays ranged from 20:1 to 50:1. Not all peptides were tested in all animals (nt).

  • b The CTPYDINQM CTL epitope had escaped at the time of the assays in 95024.