Table 6.

Summary of ELISPOT, CTL, and tetramer responses against 37 Mamu-A*01-bound SIV-derived peptidesa

Peptide no.Peptide nameSequenceELISPOTCTLTetramer
1 Env_CL8 CAPPGYAL+++
2 Pol_LV10 LGPHYTPKIV++
3Pol_EA11EAPQFPHGSSA
4 Env_CL9 CAPPGYALL+++
5Env_ST10SPPSYFQTHT+
6 Pol_SV9621 STPPLVRLV+
7 Pol_QV9 QVPKFHLPV++
8 Env_TL9 TVPWPNASL++
9Vpx_GL10GPPPPPPPGL
10Pol_IL10IYPGIKTKHL
11Pol_SL8STPPLVRL
12Pol_YI9YTPKIVGGI+
13 Gag_LF8 LAPVPIPF++
14Rev_DL10DPPTNTPEAL
15Pol_GM10GSPAIFQYTM+
16Gag_LA9LAPVPIPFA+
17Vpx_HV10HLPRELIFQV
18Env_SI9SWPWQIEYI
19Pol_MI8MTPAERLI+
20Pol_QF10QMPRQTGGFF+
21Pol_GT9GSPAIFQYT
22 Gag_CM9 CTPYDINQM+++
23Vif_LL8LTPERGWL
24Gag_VL8VVPGFQAL
25Vif_VI8VTPDYADI+
26 Vif_QA9 QVPSLQYLA++
27 Tat_TL8 TTPESANL+++
28Vif_RW9RIPERLERW
29Env_AV8AAPTSAPV+
30 Gag_QI9 QNPIPVGNI+
31Env_YI9YVPCHIRQI
32 Env_ST9 SSPPSYFQT+
33 Vpx_II11 IPPGNSGEETI+
34Gag_VT10VNPTLEEMLT+
35Env_IT9ITPIGLAPT
36 Gag_LW9 LSPRTLNAW+
37 Pol_SV9692 SGPKTNIIV+
  • a Since the Env_CL8 and Env_CL9 peptides overlap, we consider this a single positive response. Positive responses (+) in ELISPOT, 51Cr release assays, and tetramer stains are indicated. Fourteen Mamu-A*01-bound SIV-derived peptides were considered positive responders (bold) based on results from ELISPOT assays and confirmed in the majority of cases by CTL assays and tetramer staining.