Table 4.

Cell surface distribution of enveloped PRV on the surfaces of LLC-PK1 cellsa

CellsNo. of virus particles per cell
ApicalBasalCell junctionsTotal
Parental406 (13.1)242 (7.8)140 (4.5)788 (25.4)
μ1B transfectant106 (3.3)490 (15.3)320 (10.0)916 (28.6)
  • a Parental LLC-PK-1 cells or stably transfected cells expressing μ1B were grown on plastic coverslips and infected with wild-type PRV for 16 h Cells were then fixed and processed for electron microscopy as described for Table 1.