Table 3.

Distribution of virus particles in polarized MDBK and nonpolarized Vero cellsa

CellsIntracellular locationVirion structureNo. of particles/cellb
Wild-type HSV-1F-gEβ
MDBKNucleusNonenveloped27 ± 3.919 ± 5.2
CytoplasmEnveloped23 ± 3.0111 ± 7.7
Nonenveloped3.2 ± 2.67.1 ± 4.1
Cell surfaceEnveloped88 ± 9.720 ± 3.8
VeroNucleusNonenveloped35 ± 2.637 ± 3.4
Perinuclear spaceEnveloped18 ± 4.112 ± 4.2
CytoplasmEnveloped31 ± 4.235 ± 2.1
Nonenveloped5.2 ± 2.46.4 ± 3.2
Cell surfaceEnveloped102 ± 6.789 ± 8.6
  • a MDBK or Vero cells growing in plastic dishes were infected with wild-type HSV-1 strain F or F-gEβ for 20 h. Cells were then fixed with glutaraldehyde, scraped from the plastic, and processed for electron microscopy. Approximately 2,200 wild-type virus particles and 2,200 F-gEβ particles were counted in the MDBK cell samples.

  • b Data are averages with standard deviations.