Table 1.

Primers and probes used in this study

Primer or probeSequencea
oJD11 primer5′-ttggtttgaccttgcttcttgg-3′
oJD13 primer5′-ggaaataagagggtggggaagag-3′
oJD32 primer5′-cttggggatgtgcgtttag-3′
oJD33 primer5′-agaggcgtatcagttggcg-3′
A1 probe5′-(RED 640)-cttccccaccctcttatttccagca-PO4-3′
M1 probe5′-caggggtataggccagtgttc-(fluorescein)-3′
A2 probe5′-(RED 640)-tatttccagcagacccatatccaacag-PO4-3′
M2 probe5′-gtgttctcttccccaccctc-(fluorescein)-3′
  • a Bold type indicates the site of mutation.