Table 3.

Detection of Ba-L and NL4-3 proviruses in sorted thymic cells

CohortDay post-inoculationVirusMouse no.No. of proviruses/ 1,000 autofluorescent cellsaNo. of proviruses/1,000 CD4+ CD8+thymocytesaRatiob
119Ba-L125<1>5
135<1>5
145<1>5
1525<1>25
214Ba-L255<1>5
261<1>1
2725<1>25
281<1>1
NL4-3155250.2
16251000.25
514Ba-L1725<1>25
21Ba-L18551
20551
212555
NL4-3125250.25
13251250.25
1625251
  • a Calculated from the highest dilution of lysed cells which yields a detectable PCR product (for example, HIV-1 DNA could be detected by PCR in 40 but not 8 autofluorescent cells from mouse 17; thus, 40 cells must contain at least one provirus, and 1,000 cells must contain at least 25 proviruses).

  • b Ratio of the frequency of provirus in autofluorescent cells over the frequency of provirus in CD4+ CD8+ thymocytes.