Table 3.

Vectors packaged by PD223 cells efficiently transduce CHO cellsa

Packaging cellsReferenceEnvelope type or descriptionLAPSN titer (FFU/ml)bon:
CHOD17NIH 3T3
PA317 6 Amphotropic<201 × 106 NDc
PG13  8 GALV6003 × 106 ND
PT67 7 10A18003 × 105 ND
PX 1 Xenotropic<203 × 105 ND
PM571 12 MCF/polytropic<20ND1 × 105
PE501 9 Ecotropic<20ND1 × 106
FLYRD 3 RD114<204 × 106 ND
PD223This reportMDEV2 × 104 2 × 105 ND
  • a Cells were plated at 105cells per 3.5-cm-diameter well of a 6-well dish on day 1, infected on day 2 in the presence of 4 μg of Polybrene per ml, and stained for AP+ foci on day 4. All packaging cells, D17 cells, and NIH 3T3 cells were cultured in Dulbecco’s modified Eagle’s medium with 10% FBS, and CHO-K1 cells (ATCC CCL 61) were grown in α-minimal essential medium with 5% FBS.

  • b Titers are means of results from duplicate assays, which varied from the mean by no more than 18%. The experiment was repeated with similar results.

  • c ND, not determined.