Table 4.

RSV-specific CTLp frequencies for MHC class I- and II-restricted lymphocytes isolated from the spleens of BALB/c mice challenged with RSV or HPIV3 3 weeks after intramuscular immunization with FI-RSV or FI-HPIV3a

No. of days p.i.bVirusMHC classCTLp frequency rangec
PrimarySecondary
0FI-RSVI1:111,300–1:113,600
0FI-RSVII1:11,900–1:12,200
3FI-RSVRSVI1:12,600–1:14,400
3FI-RSVRSVII1:19,700–1:24,600
4FI-RSVRSVI1:2,300–1:3,900
4FI-RSVRSVII1:8,200–1:12,700
6FI-RSVRSVI1:980–1:5,700
6FI-RSVRSVII1:3,100–1:8,200
3RSVI>106
3RSVII>106
6RSVI1:4,800–1:6,000
6RSVII1:5,800–1:6,500
0FI-HPIV3I>106
3FI-HPIV3RSVI>106
5FI-HPIV3RSVI1:14,200–1:24,900d
3HPIV3I>106
5HPIV3I>106
3FI-HPIV3HPIV3I1:856,300–>106
5FI-HPIV3HPIV3I>106
  • a Three individual mice were examined at each time point for the frequencies of class I-and II-restricted CTLps, and the frequency ranges are shown.

  • b Number of days after RSV challenge.

  • c The 95% confidence limits for the CTLp frequencies shown ranged from 680 to 1,800. The limiting-dilution protocol used 51Cr-labeled, RSV-infected target cells and uninfected target cells to determine the background. The values for uninfected targets were always >1:500,000 and are not shown. The RSV-specific CTLp frequencies for age-matched control mice infected with RSV alone are shown.

  • d The 95% confidence limits for the CTLp frequencies shown ranged from 500 to 720.