Table 2.

Effect of Zta activation domain deletions on localization of BBLF4 and BBLF2/3 plus BSLF1

Localization (% of positive cells)a
NucleusNucleus + cytoplasmCytoplasm
Myc-BBLF2/3 + BSLF1 0 0100
 +wt Zta61 ± 228 ± 111 ± 2
 +Zta(Δ2-25)7 ± 45 ± 588 ± 2
 +Zta(Δ13-19)2 ± 21 ± 197 ± 3
Myc-BBLF4 0 0100
 +wt Zta91 ± 59 ± 5  0
 +Zta(Δ2-25)91 ± 19 ± 1  0
 +Zta(Δ13-19)61 ± 130 ± 19 ± 1
  • a Myc-tagged proteins were detected in transfected Vero cells by indirect immunofluorescence using anti-Myc antibody and FITC-conjugated anti-mouse Ig. Values are averages of two experiments.