Table 2.

Effect of BH1 domain mutations on the ability of Bcl-2 and Bcl-xL to bind to Beclin in the yeast two-hybrid assay

ConstructaConserved BH1 domainInhibition of apoptosis (reference)Beclin binding
WT Bcl-2 ELFRDGVNWGRIVAFFEFGG ++
WT Bcl-xL ELFRDGVNWGRIVAFFSFGG ++
MT Bcl-2 ---------A---------- − (42)
MT Bcl-xL ------AIL----------- − (5)
  • a WT, wild-type; MT, mutant.