Table 2.

WSN virus growth determined by HA assay

Fibroblast cultureVirus titera
48 h60 h72 h84 h
WT cell line<2<2<2<2
WT MEFs<2<2<2<2
STAT1−/− cell line<281632
STAT1−/− MEFs163264ND
IFNγ−/− MEFs<2<2<2<2
IFNαR−/− MEFs32646464
  • a Cell monolayers were infected with WSN virus at an MOI of 0.001 and incubated at 37°C. At the indicated time points, the amount of virus in the supernatant was determined by HA assay. At 12, 24, and 36 h p.i., titers in all cultures were <2. ND, not done.