Table 1.

Summary of yeast two-hybrid assay results

GAL4 activation domain plasmidbβ-Galactosidase reaction with GAL4 binding domain constructa
EmptyBcl-2Bcl-xLBcl-xSBaxLaminp53
Empty+
F1
 1–2563++ND
 1–1855ND
 1856–2563 (Mus Beclin 1–708)++ND
Human Beclin
 1–708++ND
 Beclin 1–450++ND
 Beclin 1–258ND
 Beclin 262–450++ND
 Beclin 451–708ND
 Beclin 1–1353ND
  • a +, positive reaction within 15 min; −, lack of positive reaction at 24 h; ND, not determined.

  • b Nucleotide positions of genes fused to the plasmid.