Table 1.

RSV-specific CTLp frequencies for MHC class I- and II-restricted lymphocytes isolated from BAL fluid of BALB/c mice at various time points post-i.n. infection with RSV or an HPIV3 controla

No. of days p.i.bVirusMHC classCTLp frequency rangec
3RSVI>106
3RSVII 106
5RSVI1:46,500–1:50,200
5RSVII1:145,000–1:162,100
7RSVI1:5,300–1:6,500
7RSVII1:22,800–1:28,500
7HPIV3I>106
10RSVI1:2,800–1:6,300
10RSVII1:25,500–1:32,200
10HPIV3I>106
14RSVI1:2,500–1:7,500
14RSVII1:12,500–1:38,000
48RSVI1:120,800–1:230,000
48RSVII1:210,500–1:>106
  • a Three individual mice were examined at each time point for the frequencies of class I-and II-restricted CTLps, and the range of frequencies is shown.

  • b Number of days after RSV infection.

  • c The 95% confidence limits for all of the CTLp frequencies shown ranged from 1,200 to 26,300. The limiting-dilution protocol used 51Cr-labeled, RSV-infected target cells and uninfected target cells to determine the background. The values for uninfected targets were always >1:500,000 and are not shown. The CTLp frequency for naive animals was >1:106.