Table 2.

Quantitation of expression and activities of fusion protein mutants

DNAF cell-surface fluorescence (%)aSyncytia (%)b
Wild type49.8 (100)810 ± 54 (100)
Cys 506 51.8 (104)366 ± 7 (45)
Cys 518 37.4 (75)12 ± 4 (1.5)
Cys 519 43.3 (86.8)513 ± 32 (63)
Cys 520 48.0 (96.2)755 ± 15 (93)
Cys 524 41.3 (83)785 ± 5 (97)
  • a Values are presented as the percentage of positively stained cells within the gated area of live cells.

  • b Number of syncytia (>4 nuclei) per 2.5 × 105 transfected MOLT4 cells. The values are means ± standard deviations from three separate experiments.