Table 1.

Palmitoylation of fusion protein mutants

F protein[3H]palmitic acida,b(cpm)[35S]Met and Cysb,c (relative optical density)3H/35S (%)
Wild type312 ± 781.65 ± 0.02189 (100)
Cys 506 52 ± 131.30 ± 0.0340 (21)
Cys 518 122 ± 300.88 ± 0.08139 (73)
Cys 519 237 ± 591.31 ± 0.04182 (96)
Cys 520 244 ± 611.14 ± 0.03214 (113)
Cys 524 263 ± 661.72 ± 0.02153 (81)
  • a Values represent the specific radioactivity incorporated into F0 and F1 by 4 × 106 transfected MOLT4 cells labeled for 3 h with 1 mCi of [3H]palmitic acid. The proteins recognized by anti-F MAb Ost-2 were separated by SDS-PAGE, transferred to polyvinylidene difluoride membranes, and measured on a β-radiation scintillation counter.

  • b Values are presented as means ± standard deviations from three separate experiments.

  • c Densitometric values of F0 and F1 from SDS-PAGE autoradiography of 2 × 106 transfected MOLT4 cells labeled with TRAN35S-label after radioimmunoprecipitation assay with anti-F MAb Ost-2.