Table 1.

Effect of point mutations at the 5′ end of vRNA on polymerase binding, transcription initiation, and polyadenylation

PositionResulta in the following assay:
Polymerase binding (4)Transcription initiation (5)Polyadenylation (this study)
1′  A→U±+
2′  G→Ub
3′  U→A
4′  A→U+++
5′  G→U+++
6′  A→U+++
7′  A→U
8′  A→U
9′  C→A
10′ A→U+++
11′ A→U
12′ G→U+ND
13′ G→U+
  • a +, the base change was tolerated; −, the base change was not tolerated; ±, partially tolerated; ND, not determined.

  • b An RNA template with a G→C mutation was studied in the [α-32P]ATP incorporation assay.