Table 2.

RT-PCR for transcripts from different EBNA1 promoters in cell lines and tumors

Cell lines and tumorsRT-PCR signala
Non-EBNA1-coding transcript FUU′ (Fp)EBNA1-coding transcripts
FUK (Fp)QUK (Qp)YUK (Cp/Wp)
EBV(+) cell lines
 BL cell lines
  Rael++++
  Akata++++++
  Chep+++++
  Wan++++++++
  Wewak+++++++
  Namalwa++++c ++
  Rael-AzaC++++++++
  Raji+b ++++c ++
  AG876++++++++
 LCL
  IB4++++c ++
  B95-8++++++++
EBV(+) tumors
 eBL
  BL1+/−++
  BL2+
  BL6++
  BL7++
  BL8++
  BL9++c
  BL10+++/−
 PTLD
  PTLD1+/−+/−++
  PTLD2+/−++
 NPC
  NPC1+/−+/−++
  NPC2+/−++
  NPC3++NA
  NPC4++NA
 NL
  NK1++
  NK2++
  NTL++
  • a Scored as described in Materials and Methods. NA, not available because of lack of RNA.

  • b Positive for U2′ primer but not U′ primer.

  • c Weakly positive or negative for QUK but positive for Q′UK.