Table 1.

Summary of syncytium induction by FIV envelope glycoprotein in human, feline, and rodent cell linesa

Cell lineSyncytium induction resulting fromb:
Transfection of CF1 or CT5Infection with CT5Δpol(VSV-G)Coculture with 3201-FIV cells
U87MGNoneNoneNone
SK-N-MCNoneNoneNone
3T3NoneNoneNone
208FNoneNoneNone
CrFK+++
HOS+NDNone
CHONoneNDNone
U87MG.CXCR4++++++++++++
SK-N-MC.CXCR4++++++++++++
3T3.CXCR4++++ND++++
208F.CXCR4++ND++
CrFK.CXCR4++++++++++++
HOS.CXCR4++++ND++++
HeLa++++++++++++
293++++ND++++
293T++++ND++++
Molt4 clone 8NDND++++
SupT1NDND++++
H9NDND++++
  • a See Fig. 4 for representative photographs and text for quantitative scoring of syncytia, details of transfection, infection with replication-defective CT5Δpol(VSV-G), and number of experiments performed. Transfection efficiency controls are described in Materials and Methods.

  • b ++++, syncytia too numerous to count, or lytic destruction of monolayer; ++, >5 syncytia containing more than 8 nuclei each per cm2; +, <5 such syncytia per cm2; None, no synctia seen; ND, not done.