Table 2.

Cytokine production by T cells recruited into the lungs of wild-type and IFN-γ−/− mice infected with the WSN virusa

Mouse strain and in vitro stimulationCytokine production (pg/ml)b
IFN-γIL-4IL-2
BALB/c
 ConA245 ± 42127 ± 25181 ± 39
 PR8 (H1N1) virus82 ± 321 ± 641 ± 4
 HK (H3N2) virus106 ± 2049 ± 1131 ± 5
IFN-γ−/−
 ConA022 ± 8259 ± 10
 PR8 (H1N1) virus09 ± 125 ± 5
 HK (H3N2) virus08 ± 330 ± 4
  • a Effector cells obtained by collagenase digestion of lungs harvested at day 3 after infection with the WSN virus were depleted of adherent cells and stimulated in vitro with live viruses or ConA (see Materials and Methods). The cells from three mice in each group were pooled. Cytokines were measured by ELISA after 4 days of incubation.

  • b Results are expressed as means ± SE of triplicates.