Table 2.

Sequence changes of 25 BLV LTR mutants made by site-directed mutagenesis

NameaSite of mutationbOriginal sequenceMutated sequence
d-CRE1−157 to −153AGACGDeleted
d-CRE2−131 to −129GACDeleted
d-CRE3−57 to −54TGACDeleted
s-CRE3−56GT
d-CRE 1/2−157 to −153AGACGDeleted
−131 to −129GACDeleted
d-CRE 1/3−157 to −153AGACGDeleted
−57 to −54TGACDeleted
dCRE 1/s-GRE−157 to −153AGACGDeleted
−71 to −60ACA---TG-CTTAT---AC--TA
dCRE2/s-GRE−131 to −129GACDeleted
−71 to −60ACA---TG--CTTAT---AC--TA
d-CRE2/3−131 to −129GACDeleted
−57 to −54TGACDeleted
d-CRE3/a-GRE−57 to −54TGACDeleted
−71 to −72Wild typeTATCC added
d-CRE1/2/3−157 to −153AGACGDeleted
−131 to −129GACDeleted
−57 to −54TGACDeleted
d-CRE1/2/s-GRE−157 to −153AGACGDeleted
−131 to −129GACDeleted
−71 to −60ACA---TG--CTTAT---AC--TA
d-CRE2/3/a-GRE−131 to −129GACDeleted
−57 to −54TGACDeleted
−71 to −72Wild typeTATCC added
d-CRE1/2/3/a-GRE−157 to −153AGACGDeleted
−131 to −129GACDeleted
−57 to −54TGACDeleted
−71 to −72Wild typeTATCC added
s-GRE−71 to −60ACA---TG--CTTAT---AC--TA
s-167−167AG
s-AP4-1−151GT
s-142,3−142 to −143AGCT
s-140−140AG
s-AP4-2−124GT
s-116−116CA
s-107,8−107 to −108CCAT
s-99−99CT
s-83,4−83 to −84TCCT
s,a-TATA−41 to −40TAAT
−37 to −38Wild typeT added
  • a Abbreviations: a, addition; d, deletion; s, substitution; AP4, activating protein 4.

  • b Positions of sequence changes are indicated by the numbers which represent the distance (in base pairs) relative to the RNA start site (+1).