Table 1.

Summary of mass analysis data

Variant proteinFormula mass (Da)aMean mass (Da) ± SD by:
Mass spectrometrybGel filtrationcSTEM (no. of molecules)
gD1(306t)34,92837,200 ± 20064,100 ± 8,55070,900 ± 15,200 (309)
gD2 (306t)34,93336,200 ± 20062,00065,900 ± 14,500 (262)
gD1 (QAAt)34,90934,200 ± 20065,40079,100 ± 21,100 (31)
gD1 (Δ290-299t)34,43036,400 ± 20067,700 ± 10,500 NDd
  • a Based on amino acid composition; mass of N-CHO not included.

  • b Error estimated from broadness of peaks due to sugar heterogeneity.

  • c Standard deviations derive from results of at least three experiments. The large errors in molecular mass determinations by gel filtration were due largely to the broadness of the peaks caused by the presence of N-CHO.

  • d ND, not done.