Table 2.

Oligonucleotides used in mutagenesis

Oligo- nucleo- tideSequenceCoordinates (nt)
YUAO15′-GGCTTCTCCGTCTACCGG-3′91963–91970
YUAO25′-CTCGCGCTCCCTAGGTGC-3′92892–92909
YUAO35′-CACGCATACGCCGTATGTCCGGAATTCC-3′92209–92265
YUAO45′-GGACATACGGCGTATGCGTGCGTCATCT-3′92217–92273
YUAO75′-AACGGACTGATCAGAGATGATTTCCGCC-3′92277–92332
YUAO85′-TCATCTCTGATCAGTCCGTTTTACGTAT-3′92284–92340
YUAO95′-GCCCCTACGGCGTAAAACGGACTGATGA-3′92320–92370
YUAO105′-CCGTTTTACGCCGTAGGGGCGGAGCCTA-3′92328–92378
YUAO115′-TATGCATCCTGGCGTCGCCTAGCATCCG-3′92343–92400
YUAO125′-AGGCGACGCCAGGATGCATACCCTATAT-3′92351–92408
YUAO135′-AACGGACTGACCGTATGTCCGGAATTCC-3′92209–92332
YUAO145′-GGACATACGGTCAGTCCGTTTTACGTAT-3′92217–92340
YUAO155′-GTTTCGTTAGCTGCAGATGCATCCTAGGGGTGG-3′92383–92430
YUAO165′-TAGGATGCATCTGCAGCTAACGAAACGTTCTAC-3′92390–92437
YUAO175′-GGTTCCCGTTCTGCAGCGTAGAACGTTTCGTTA-3′92422–92463
YUAO185′-ACGTTCTACGCTGCAGAACGGGAACCACCGTAA-3′92429–92470
YUAO195′-CGGAGGAGAACTGCAGTTACGGTGGTTCCCGTT-3′92454–92502
YUAO205′-ACCACCGTAACTGCAGTTCTCCTCCGGAACCGG-3′92461–92509
YUAO215′-GTAAAAATTTCTGCAGTTCCGGAGGAGAAGGGG-3′92489–92526
YUAO225′-TCCTCCGGAACTGCAGAAATTTTTACCAAATTT-3′92496–92533
YUAO235′-ATGGTTGCCCCTGCAGGCCCCCCCCGGTTCCGG-3′92500–92543
YUAO245′-CGGGGGGGGCCTGCAGGGGCAACCATGATTTCC-3′92507–92550
YUAO255′-GACTGCGCATCTGCAGGGTTGCCCAAATTTGGT-3′92525–92583
YUAO265′-TTGGGCAACCCTGCAGATGCGCAGTCGGGGCGA-3′92532–92590
YUAO295′-TGTAACCTGAAACCGCCGTG-3′91381–91400
YUAO305′-CCGTATGTCCGGAATTCCAC-3′92207–92226
YUAO315′-GACGTTGGCACTGCAGCGATCGCCACATTCGAT-3′91481–91707
YUAO325′-GTGGCGATCGCTGCAGTGCCAACGTCATAATCA-3′91488–91714
YUAO335′-ATATGGCTACCTGCAGTGCCTGTTCTTATGCCG-3′91581–91807
YUAO345′-AGAACAGGCACTGCAGGTAGCCATATCCGCTTA-3′91588–91814
YUAO355′-CGTACAAGGGCTGCAGTCTGGCACCGCCTCTTG-3′91681–91907
YUAO365′-CGGTGCCAGACTGCAGCCCTTGTACGGAAATTT-3′91688–91914
YUAO375′-AGCGTCTACGCTGCAGACGTAATGGGTGTGGCT-3′91781–92007
YUAO385′-CCCATTACGTCTGCAGCGTAGACGCTACTCCCG-3′91788–92014
YUAO395′-ACAGAGGAAGCTGCAGGTGGAGTCTAGGGAGGG-3′91881–92107
YUAO405′-TAGACTCCACCTGCAGCTTCCTCTGTTTTGGCC-3′91888–92114
YUAO415′-TCCGGAATTCCTGCAGTCAGCAGGTGTATATTT-3′91981–92219
YUAO425′-CACCTGCTGACTGCAGGAATTCCGGACATACGG-3′91988–92226
YUAO435′-GACTGCGCATCTGCAGATGCATCCTAGGGGTGG-3′92383–92583
YUAO445′-TAGGATGCATCTGCAGATGCGCAGTCGGGGCGA-3′92390–92590
926545′-CGGCGCATGCGCACTCGAGT-3′92635–92654
RP15′-TTCTAGAACCGCTGGATGCA-3′
RP25′-TCCAGCGGTTCTAGAATGCA-3′
9RP15′-TTCTAGACGCGATGCA-3′
9RP25′-TCGCGTCTAGAATGCA-3′