Table 1.

Rescue of ts10 by NPH-II alleles containing alanine mutations in motifs II and III

NPH-II geneVirus titer at 40°C (PFU/ml)Sp activity of protein (% of wild-type activity)
ATPaseHelicase
Wild type1 × 107 100100
D296A2 × 104 5 a <5 a
E297A3 × 104 5 a <5 a
H299A3 × 104 58<1
E300A1 × 104 1<1
T326A1 × 104 51<1
T328A1 × 104 521
Plasmid<10
  • a From reference 8.