Table 4.

Cytokine secretion following adoptive transfer of virally infected APCs into animals transduced intramuscularly with AAVlacZa

GroupLeft legAdoptive transferIFN-γ (pg/ml)IL-10 (pg/ml)
MockAd5β-GalAAVMockAd5β-GalAAV
5AAVlacZ APC AdlacZ 113423418265548501,020230
6AAVlacZ APC naive1.54.5709.224236659
7AAVlacZ APC AdBglII342198012054813340320
8AAVlacZ APC AAVlacZ 23.45.89.2343570160
9AAVlacZ APC AAVlacZ/AdBglII51195929557605291214
  • a Lymphocytes from regional lymph nodes and spleen were harvested 10 days after intramuscular infection with AAVlacZ and adoptive transfer of 5 × 106APCs infected ex vivo with AdlacZ (group 5), AdBglII (group 7), AAVlacZ (group 8), or AAVlacZ plus AdBglII (group 9) and mock infected (group 6). Cells were restimulated with purified β-galactosidase (β-Gal), AAVlacZ (AAVlacZ), UV-inactivated adenovirus type 5 (Ad5), or medium (Mock) for 48 h, and the supernatants were assayed for the secretion of IL-10 and IFN-γ by cytokine ELISA. A representative experiment is shown; this study was repeated twice with identical results.