Table 1.

Overview of experimental strategya

GroupLeft legRight legGroupLeft legAdoptive transfer
1AAVlacZ 5AAVlacZ APC AdlacZ
2AAVlacZ AdlacZ 6AAVlacZ APC naive
3AAVlacZ +   AdBglII7AAVlacZ APC AdBglII
4AAVlacZ+   AdALP 8AAVlacZ APC AAVlacZ
9AAVlacZ APC AAVlacZ/    AdBglII
10AAVlacZ APC AdlacZ/    AAV ApoE
  • a Animals were injected with AAVlacZ into the left tibialis anterior alone (group 1) and either coinjected into the same muscle with an E1-deleted adenovirus not expressing any transgene (i.e., AdBglII [group 3]) or expressing the ALP transgene (group 4), or injected separately with an adenovirus expressing the lacZ transgene into the right leg (group 2). The second set of experiments (groups 5 to 9) were based on adoptive transfer into C57BL/6 mice of 5 × 106 splenic APCs infected with various combinations of vectors ex vivo; all animals were injected with AAVlacZ in the left tibialis anterior at the time of adoptive transfer. These include APCs infected with AdlacZ (group 5), AdBglII (group 7), AAVlacZ (group 8), AAVlacZ and AdBglII (group 9), and AdlacZ and AAV ApoE (group 10). Group 6 received nontransduced APCs. The left tibialis anterior was harvested 10, 28, or 60 days later, cryosectioned, and stained for β-galactosidase activity.